Gene expression underlying ferret hair growth initiation induced by melatonin and modified by steroids

Synchronised ferret winter pelage development induced by melatonin was utilised as an in vivo model for studying gene expression associated with the hair growth cycle. The effects of melatonin and steroids on hair follicle growth initiation in flank skin samples were monitored by histology and immunocytochemistry. Melatonin implantation initiated hair growth within 10 days. Exogenous steroids delivered by slow-release subcutaneous implants had mainly inhibitory effects on spontaneous and melatonin-initiated hair growth, either when given simultaneously or prior to melatonin administration. 17β-oestradiol totally suppressed hair follicle growth in all circumstances, while the inhibitory effects of dexamethasone, testosterone and deoxycorticosterone were weaker and attenuated by melatonin. Progesterone given simultaneously with melatonin inhibited hair growth in males, but did not significantly affect female hair growth initiated by melatonin. Gene expression underlying ferret hair growth was studied using an optimised differential display technique using two sets of complementary skin samples covering all growth initiation stages. The differential display band patterns for approximately 8% of all skin transcripts suggested that more than a thousand genes are required for hair follicle growth. Of the differentially expressed genes detected in a set of flank skin samples covering all proanagen stages, up to a half are likely to be specifically associated with hair growth. The 150 expressed sequence tags (ESTs) discovered represented 112 unique sequences. Forty-two of these were aligned by sequence homology to known genes, while the remainder are likely to represent novel genes. Differential expression through the follicle growth cycle or after the treatments was confirmed for 21 out of 23 genes whose mRNAs were detectable by Northern blot hybridisation. Overall, expression alteration for most of these ESTs occurred prior to morphological changes in the skin associated with hair follicle reactivation. All the ESTs whose expression sites were detected by in situ hybridisation were localised to hair follicles. Some were detected only in certain stages of the hair follicle cycle, others in all growth stages, but at different levels. Classification of the identified ESTs suggested that apoptosis as well as fatty acid synthesis and cholesterol metabolism were closely associated with hair growth initiation, while cell interaction and motility were also particular prominent processes in hair follicle regrowth. Additional DNA sequence information was obtained for ferret hair acidic keratin 8 (fHa8) and ferret cyclin D-interacting myb-like protein 1 (fDMP1). Both of these genes were expressed only in cortical cells in the keratogenous zone from mid-proanagen. fHa8 was expressed only in one side of the cortex, a feature has not previously been reported for other type I intermediate hair keratins. The restricted expression of fDMP1 and its smaller size suggested that it was an alternatively spliced form of the human and mouse homologues with a specialised function in the regulation of cortical cell proliferation and differentiation. Further studies on the expression regulation and function of the genes represented by the ESTs identified in this study promise to considerably advance our understanding of hair follicle growth control

Video-assisted thoracic bronchial sleeve lobectomy with bronchoplasty for treatment of lung cancer confined to a single lung lobe: a case series of Chinese patients

BACKGROUND: The outcomes of video-assisted thoracic bronchial sleeve lobectomy (VABSL), a minimally invasive video-assisted thoracoscopic (VATS) lobectomy, are mostly unknown in Chinese patients. OBJECTIVES: To investigate operative and postoperative outcomes of VABSL in a cases series of Chinese patients with lung cancer. METHODS: Retrospective study of 9 patients (male:female 8:1; mean age 59.4 ± 17.6 years, ranging 21–79 years) diagnosed with lung cancer of a single lobe, treated with VABSL between March 2009 and November 2011, and followed up for at least 2 months (mean follow-up: 14.17 ± 12.91 months). Operative outcomes (tumor size, operation time, estimated blood loss and blood transfusion), postoperative outcomes (intensive care unit [ICU] stay, hospitalization length and pathological tumor stage), death, tumor recurrence and safety were assessed. RESULTS: Patients were diagnosed with carcinoid cancer (11.1%), squamous carcinoma (66.7%) or small cell carcinoma (22.2%), affecting the right (77.8%) or left (22.2%) lung lobes in the upper (55.6%), middle (11.1%) or lower (33.3%) regions. TNM stages were T2 (88.9%) or T3 (11.1%); N0 (66.7%), N1 (11.1%) or N2 (22.2%); and M0 (100%). No patient required conversion to thoracotomy. Mean tumor size, operation time and blood loss were 2.50 ± 0.75 cm, 203 ± 20 min and 390 ± 206 ml, respectively. Patients were treated in the ICU for 18.7 ± 0.7 hours, and overall hospitalization duration was 20.8 ± 2.0 days. No deaths, recurrences or severe complications were reported. CONCLUSIONS: VABSL surgery is safe and effective for treatment of lung cancer by experienced physicians, warranting wider implementation of VABSL and VATS training in China

Current Situation and Development Trend of Mobile Communication Systems

This paper introduces the development background of mobile communication and the development of mobilecommunication. It introduces the application principle, network structure, main technology, the advantages anddisadvantages of the three generations of mobile communication system respectively, and introduces the currentthird generation mobile communication system, including its technical support and research direction, analysis andcomparison of the European WCDMA system, the United States CDMA2000 system and China's TD-SCDMA systemtechnical characteristics. Finally, the development trend and prospect of future mobile communication system arediscussed

Navigating Text-To-Image Customization:From LyCORIS Fine-Tuning to Model Evaluation

Text-to-image generative models have garnered immense attention for their ability to produce high-fidelity images from text prompts. Among these, Stable Diffusion distinguishes itself as a leading open-source model in this fast-growing field. However, the intricacies of fine-tuning these models pose multiple challenges from new methodology integration to systematic evaluation. Addressing these issues, this paper introduces LyCORIS (Lora beYond Conventional methods, Other Rank adaptation Implementations for Stable diffusion) [https://github.com/KohakuBlueleaf/LyCORIS], an open-source library that offers a wide selection of fine-tuning methodologies for Stable Diffusion. Furthermore, we present a thorough framework for the systematic assessment of varied fine-tuning techniques. This framework employs a diverse suite of metrics and delves into multiple facets of fine-tuning, including hyperparameter adjustments and the evaluation with different prompt types across various concept categories. Through this comprehensive approach, our work provides essential insights into the nuanced effects of fine-tuning parameters, bridging the gap between state-of-the-art research and practical application.Comment: 77 pages, 54 figures, 6 table

Computational prediction of novel non-coding RNAs in Arabidopsis thaliana

BackgroundNon-coding RNA (ncRNA) genes do not encode proteins but produce functional RNA molecules that play crucial roles in many key biological processes. Recent genome-wide transcriptional profiling studies using tiling arrays in organisms such as human and Arabidopsis have revealed a great number of transcripts, a large portion of which have little or no capability to encode proteins. This unexpected finding suggests that the currently known repertoire of ncRNAs may only represent a small fraction of ncRNAs of the organisms. Thus, efficient and effective prediction of ncRNAs has become an important task in bioinformatics in recent years. Among the available computational methods, the comparative genomic approach seems to be the most powerful to detect ncRNAs. The recent completion of the sequencing of several major plant genomes has made the approach possible for plants.ResultsWe have developed a pipeline to predict novel ncRNAs in the Arabidopsis (Arabidopsis thaliana) genome. It starts by comparing the expressed intergenic regions of Arabidopsis as provided in two whole-genome high-density oligo-probe arrays from the literature with the intergenic nucleotide sequences of all completely sequenced plant genomes including rice (Oryza sativa), poplar (Populus trichocarpa), grape (Vitis vinifera), and papaya (Carica papaya). By using multiple sequence alignment, a popular ncRNA prediction program (RNAz), wet-bench experimental validation, protein-coding potential analysis, and stringent screening against various ncRNA databases, the pipeline resulted in 16 families of novel ncRNAs (with a total of 21 ncRNAs).ConclusionIn this paper, we undertake a genome-wide search for novel ncRNAs in the genome of Arabidopsis by a comparative genomics approach. The identified novel ncRNAs are evolutionarily conserved between Arabidopsis and other recently sequenced plants, and may conduct interesting novel biological functions

Analysis of seismicity characteristics in southern Jiangxi and neighboring areas

In this paper, the characteristics of time, space, strong and activity frequency, b value, strain energy release of moderate earthquakes in southern Jiangxi and neighboring areas (23.66°N～27.20°N, 113.50°N～118.84°E) areanalyzed. The results show that, the region has the background of moderate and strong seismicity, and modern small earthquakes are distributed in dense belts. The main aftershock type and swarm type are the main earthquake sequences above moderate strength. Since 1971, seismic activity has continued to be active, with earthquakes of ML≥4.0 exhibiting obvious clustering property, mainly manifested by the spatial and temporal concentration of seismic activity; The seismicity of ML≥3.5 earthquakes has periodic characteristics, including the high active period, the quiet period and the occurrence of destructive earthquake events; When the b value is always lower than the annual average value, the moderate and strong earthquakes are active; The rate of strain energy release curve shows an accelerating trend before moderate earthquakes are active. The results of this study provide some important reference information for predicting the time, space and intensity of earthquakes in this region, which helps to closely observe the seismic activity in the region